가이드북 & Key Visual
Cell Cycle Analysis Kitwithout Cell Pre-Fixation
The cell cycle, being directly responsible for cell proliferation, is a major target for various therapies. Cell cycle analysis is therefore an important tool for studying not only how cells proliferate, but also the therapeutic mechanisms underlying its regulation. The classic cell cycle assay requires cells to be pre-fixed with ethanol and treated with RNase, before finally being stained with DAPI or propidium iodide and analyzed. Depending on the cell line being used and the needs of the experimenter these extra steps can take anywhere from several hours to an entire day. However, the Cell Cycle Assay Kit (Red) (Product No. C3851) uses a special surfactant to permeabilize the cell membrane just enough for a DNA-specific fluorescent dye (7-AAD, λex, max = 549nm, λem, max = 648nm) to enter without DNA leaving the cell. In this way, analysis of the cell cycle can be performed without pre-fixation. Just collect your cells-of-interest, incubate with the included buffer / dye for as little as 15 minutes, and measure.
Product
C3851 Cell Cycle Assay Kit (Red)
7-AAD for 100 assays
Cell staining buffer for 100 assays
첨부파일 |
---|