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[Product Highlight] The Decoloring Solution for Plant Tissue-Clearing Method iTOMEI
TCI Product Highlight에서는 TCI의 특정 제품에 초점을 맞춰 해당 제품의 특성과 사용 방법을 소개하고 있습니다.
[20240311]
The Decoloring Solution for Plant Tissue-Clearing Method iTOMEI
Tissue-Clearing Reagent iTOMEI-D [for Plants] (1) is a decoloring solution used for the improved TOMEI method (iTOMEI), a transparency method that is an improved version of the TOMEI method developed by Matsunaga et al.1,2) iTOMEI has the advantage of making plants transparent while suppressing the quenching of fluorescent proteins such as GFP and tdTomato. Plants that have been fixed in 1% paraformaldehyde / PBS can be immersed in the decoloring solution 1 to remove chlorophyll from plants. The transparent plants can then be observed by treating them with a mounting solution that matches the refractive index of the objective lens to make the refractive index uniform.
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References
- 1) Improved clearing method contributes to deep imaging of plant organs
- 2) Whole-Tissue Three-Dimensional Imaging of Rice at Single-Cell Resolution
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